Cooperation in signal transduction of extracellular guanosine 5’ triphosphate and Nerve Growth Factor in neuronal differentiation of PC12 cells
Articolo
Data di Pubblicazione:
2004
Abstract:
We studied the transduction mechanisms activated by GTP in PC12 cells and found that addition of GTP (100 uM) increased intracellular calcium concentration ([Ca2+]i) in cells that were between 60 and 70% confluent. Addition of GTP also enhanced activation of NGF-induced extracellular regulated kinases (ERKs) and induced Ca2+ mobilization.
This mobilization, due to the activation of voltage-sensitive and ryanodine-sensitive calcium channels, as well as pertussis toxin-sensitive purinoceptors, modulates Ca2-activated K+ channels not involved in activation of ERKs. The results presented here indicate that GTP-triggered [Ca2+]i increase may be a key event in GTP signal transduction, which can modulate activity of ERKs. The physiological
importance of the GTP effect lies in its capacity to interact with the NGF-activated pathway to enhance neurite outgrowth from PC12 cells.
Tipologia CRIS:
1.1 Articolo in rivista
Elenco autori:
Guarnieri, Simone; FANO' ILLIC', Giorgio; Rathbone, Mp; Mariggio', Maria Addolorata
Link alla scheda completa:
Pubblicato in: