Evaluation of the Effectiveness of an Innovative Polycomponent Formulation on Adult and Aged Human Dermal Fibroblasts
Articolo
Data di Pubblicazione:
2023
Abstract:
Skin aging is a dynamic process that determines structural alterations in ECM and reduction in dermal fibroblasts. The recent availability on the market of an innovative polycomponent formulation (KARISMA Rh Collagen® FACE, K) containing non crosslinked high-molecular-weight hyaluronic acid (HMW-HA), a human recombinant polypeptide of collagen-1 alpha chain, and carboxymethyl cellulose (CMC), attracted our scientific interest in evaluating its biomolecular effects on human dermal adult and aged fibroblasts. After treatment with increasing K concentrations, cell proliferation, collagen I, prolyl 4-hydroxylase (P4HA1), an essential protein in collagen biosynthesis, and alfa-SMA levels were assessed. The fibroblast contractility, TGF-beta 1 levels, and oxidative stress markers were also evaluated. K formulation exposure led to a significant and dose-dependent increase in the proliferation and migration of adult fibroblasts. Of note, the K exposure counteracted the H2O2-induced aging by promoting cell proliferation, reducing beta-galactosidase activity, and neutralizing the aging-associated oxidative damage. Moreover, an increase in collagen I, P4HA1, alpha-SMA, TGF-beta 1 levels, and improved contractility of adult and aged fibroblasts were observed after treatment. Overall, our results show evidence that the K treatment is efficacious in improving
biological functions in adult fibroblasts and suppressing the biomolecular events associated with H2O2-induced cellular aging, thus supporting the regenerative and bio-revitalizing action of the K formulation helpful in preventing or treating skin aging.
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
polycomponent formulation; skin aging; dermal fibroblast; human recombinant polypeptide
of collagen-1 alpha chain; TGF-beta 1; oxidative stress
Elenco autori:
Augello, Fr; Lombardi, F; Artone, S; Ciafarone, A; Altamura, S; Di Marzio, L; Cifone, Mg; Palumbo, P; Giuliani, M; Cinque, B
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