Development of Bio-mimetic Synthetic nano-VEsicles as intelligent delivery strategy for tendon therapeutic application
Progetto This proposal aims at mimicking the physiological mechanisms involved in
promoting the tenogenesis process through the inductive
influence of bioactive molecules administered via extracellular vesicles
(EVs). These EVs are generated by Tendon Progenitor Stem
cells during their tenogenic commitment events and will be delivered by
using bio-mimetic synthetic nano-vesicles, intended as
novel therapeutic nanomedicine approach for tendon regeneration.
In detail, these main goals will be pursued according to a specific
experimental protocol encompassing the three following steps:
i. Characterization of Tendon Stem/Progenitor Cells (TSPCs) isolated from
human surgery explants and study of the tenogenic
activity of their EVs;
ii. Preparation of synthetic nano-vesicles using the same membrane lipids
composition of TSPCs-derived EVs (Bio-sVE);
iii. In vitro evaluation of the tenogenic cell-instructive effect of Bio-sVE
adopting a biomimetic environment assembled with a 3D
scaffold bioengineered with human stem cells cultured under cyclic strain in
a bioreactor system.
Advanced pharmaceutical nanotechnologies for drug delivery opens
important perspectives for pharmaceutical industries of
strategic strengths for the European economy, as evidenced by the EUHorizon founding programs, the National Plan for Research
(2021-27), and the National Recovery and Resilience Plan included in the
Next Generation EU programme. The impact of the
collected data will allow to prototype Bio-sVE for the controlled delivery of
teno-regenerative molecules capable of improving the functional recovery of
injured tissues.