ID:
BIO11A
Duration (hours):
24
CFU:
3
SSD:
BIOLOGIA MOLECOLARE
Located in:
CHIETI
Url:
BIOTECNOLOGIE AVANZATE /GENOMICA APPLICATA Year: 2
Year:
2025
Course Catalogue:
Overview
Date/time interval
Secondo Semestre (01/03/2026 - 12/06/2026)
Syllabus
Course Objectives
The course aims to provide students with advanced theoretical knowledge and practical skills in molecular biology, gene regulation, and genetic manipulation technologies, preparing them to work in scientific research, diagnostics, biotechnology, and the pharmaceutical industry.
By the end of the course, students will be able to understand the molecular mechanisms of gene regulation, design and conduct experiments, analyze and interpret experimental data, communicate scientific results clearly and coherently, and independently keep up to date with new discoveries and techniques in molecular and applied biology.
Knowledge and UnderstandingStudents will acquire in-depth knowledge of gene expression regulation in vertebrates, including genome organization, RNA types (messenger RNA, small non-coding RNA, long non-coding RNA), transcription, post-transcriptional regulation, epigenetics (DNA methylation and histone modifications), and diagnostic applications of gene expression analysis.
Applying Knowledge and UnderstandingStudents will be able to design and perform experiments to analyze gene expression, identify regulatory factors using bioinformatic and experimental techniques (ChIP, RIP, RNA pull-down, reporter genes), employ molecular technologies (qPCR, digital PCR, expression arrays, CRISPR), and interpret experimental data with scientific rigor.
Making JudgmentsStudents will be able to critically evaluate experimental results and scientific literature, identify the limitations and potential of gene analysis methods, formulate independent hypotheses and experimental strategies, and develop problem-solving skills in molecular research contexts.
Communication SkillsStudents will be able to communicate concepts, methods, and experimental results clearly and coherently, both orally and in writing, preparing laboratory reports, scientific presentations, and posters, using appropriate technical terminology and participating effectively in scientific discussions.
Learning SkillsStudents will be able to independently update their knowledge on new techniques and discoveries in molecular biology and gene regulation, integrate theoretical and practical knowledge in research contexts, and develop independent study and experimental strategies to address complex problems.
Teaching Methods
Teaching activities will be divided into lectures and laboratory exercises.
The lectures last 2 hours each and include a 10-15 minute break at the end of the first hour. Students are warmly invited to ask questions both during and at the end of the lectures.
The final part of the course will be devoted to laboratory exercises on molecular biology methods covered in the lectures.
Assessment Methods
Learning assessment will take place at the end of the course, during the scheduled exam sessions, through an oral examination in the form of an interview, covering multiple topics from the program.
The outcome of the exam is expressed on a scale of thirty points. Assessment takes into account the level of knowledge demonstrated, the appropriateness of the scientific language used, as well as the clarity, coherence, and logical rigor of the exposition.
Assessment Criteria
The exam is graded on a thirty-point scale according to the following levels:
18–22/30: sufficient basic knowledge, though with gaps;
23–25/30: fair knowledge accompanied by clear exposition;
26–28/30: good command of the subject and ability to present topics using appropriate language;
28–30/30: very thorough knowledge, excellent exposition skills, and critical reasoning.
Honors (cum laude) are awarded to students who demonstrate particularly advanced knowledge, outstanding presentation skills, and strong critical thinking abilities.
Texts
BIOLOGIA MOLECOLARE; Casa Editrice Ambrosiana
Francesco Amaldi Piero Benedetti Graziano Pesole
Paolo Plevani
FONDAMENTI DI BIOLOGIA MOLECOLARE
Lizabeth A. Allison
Seconda edizione italiana condotta sulla terza
edizione americana
BIOTECNOLOGIE MOLECOLARI
Terry A. Brown
Principi e tecniche Terza edizione italiana condotta
sull'ottava edizione inglese
RNA MethodologiesA Laboratory Guide for Isolation and Characterization6th Edition - November 23, 2022Academic PressAuthor: Robert E. Farrell Jr.Language: EnglishPaperback ISBN: 9780323902212
Contents
The module provides an in-depth examination of genome organization and structure in vertebrates, with particular emphasis on the distinction between protein-coding genes and non-coding RNAs and their roles in fundamental cellular processes. The mechanisms of transcription and the main levels of gene expression regulation, including both transcriptional and post-transcriptional stages, are analyzed.
Significant attention is given to the experimental approaches used to study gene regulation, with a focus on molecular analysis techniques, epigenetic mechanisms, and DNA–protein interactions.
Finally, the module emphasizes the use of gene expression analysis as an advanced diagnostic tool, highlighting its importance in the detection and characterization of diseases.
Course Language
Italian
More information
Student reception times are to be agreed with the lecturer by e-mail (michele.sallese@unich.it).
Degrees
Degrees
BIOTECNOLOGIE AVANZATE
Master’s Degree
2 years
No Results Found
People
People
Docenti di ruolo di IIa fascia
No Results Found