TE208 - ANALYTICAL CHEMISTRY

courses

ID:

TE208

Duration (hours):

52

CFU:

SSD:

CHIMICA ANALITICA

Located in:

CHIETI

Url:

Course Details:

SUSTAINABLE TECHNOLOGIES AND ENVIRONMENTAL TOXICOLOGY/CORSO GENERICO Year: 1

Year:

2025

Course Catalogue:

https://unich.coursecatalogue.cineca.it/af/2025?co...

Date/time interval

Secondo Semestre (01/03/2026 - 15/06/2026)

Course Objectives

The Analytical Chemistry course includes 6 CFUs divided as follows: 5 CFU of lectures and 1 CFUs in the laboratory The aim of the course is to guide and train the student regarding theoretical and practical aspects of analytical chemistry. The course, divided into lessons and exercises, will deal with basic but fundamental aspects of analytical chemistry and related exercises

Course Prerequisites

Students must have acquired the basic notions of General and Inorganic Chemistry

Teaching Methods

The Analytical Chemistry module includes 5 CFU of lectures and 1 CFU in the laboratory where the various topics will be addressed and which will find enrichment in laboratory lessons in order to deepen the technical and manual skills.

Assessment Methods

The exam of the Analytical Chemistry module consists of a written test lasting 120 minutes in which 5 exercises will be proposed. The exercises may also include theory questions. This test allows you to achieve a maximum score of 30. If the score is less than 15, the test is considered insufficient. The oral exam is an element of evaluation and the final grade will be established taking into account the scores achieved in the two tests.

Texts

Skoog, D.A., West, D.M. Holler, F.J. Crouch: S.R. - Fondamenti di Chimica Analitica, EdiSes,

ANALYTICAL CHEMISTRY
Finalities of Analytical Chemistry. Phases of the chemical-analytical procedure. Classification of analysis methods. Processing of experimental data. Average value of a series of data, mode and median. Standard deviation and relative standard deviation. Systematic, random and gross errors. Absolute error and relative error. Source and treatment of systematic errors in analytical chemistry. Statistical treatment of random error. Probability distributions of Gauss and Student's t. Confidence intervals of the mean. Accuracy and precision. laboratory exercises. Mass spectrometry and its applications in the environmental field in the determination of xenobiotics (LC-MS, LC-MS/MS). ICP-MS. Introduction to chromatographic separations: general principles. Classification of chromatographic methods. Chromatographic parameters: retention time, retention and selectivity factor, resolution. Efficiency of a chromatographic column: number of theoretical plates, equivalent height of a theoretical plate. van Deemter equation. Band widening. Variables affecting chromatographic efficiency. Qualitative and quantitative analysis by chromatography. Exercises. Principles of gas chromatography (GC). Isocratic and gradient chromatography. High and ultra-performance liquid chromatography (HPLC, UPLC), instrumentation and detectors.

Sample preparation techniques for the determination of inorganic and organic analytes, of pollutants in the determination of heavy metals, pesticides, aromatic compounds, organic solvents. Air pollutants. Emerging pollutants, Drugs (antibiotics, anticancer drugs, antidepressants, hormones) and their metabolites. Endocrine modulators, plasticizers, PCBs. Personal care products. Fragrances, insecticides, glyphosate. Microplastics, metal nanoparticles. Polluting mixtures.
Electromagnetic radiation. Origin of the emission and absorption spectra. Sources, monochromators and radiation detectors. Visible/UV molecular absorption spectrophotometry. The absorption spectra of molecules. Relationship between absorption and molecular structure. Lambert-Beer law. Spectrophotometers and their calibration. Spectrophotometric analysis of a single substance. Analysis of mixtures. Spectrophotometric titrations. Luminescence and Chemiluminescence. Fluorescence and phosphorescence. Fluorimetry. Exercises. Atomic spectroscopy: general aspects. Atomization systems. Quantitative relations and calibration lines. Emission spectroscopy. Atomic Absorption Spectroscopy (AA) on flame and electroatomizer. Atomic emission with plasma atomizers.