Physicochemical properties of titanium surfaces, such as wettability, influence protein binding, cell adhesion and proliferation, therefore osseointegration. The objective of this study was to investigate the wetting behaviour of two titanium surfaces, sandblasted and double acid etched (group S/E) and sandblasted (group S), using blood and Autologous Platelet Liquid (APL). Surface morphology and roughness were investigated by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The static contact angle (CA) was assessed with the sessile drop technique. The work also evaluates, with SEM observation, the fibrin clot structure that develops from blood and APL, knowing that a greater clot, firmly attached to an implant can facilitate cell migration to the implant interface. Both surfaces exhibited a hydrophobic behaviour, regardless of the wetting liquid used, but the S surface showed higher CA values for both the wetting fluids used. Lower CA values on the S/E surface are attributable to the different surface energy, which depends on different surface topography (the S surfaces were rougher) and on chemical composition. No statistically significant differences between the values of CA of blood and APL were found on the same surfaces. The clot obtained from whole blood differs from the APL clot due to a different cellular composition and fibrin density.