CROC2.0 is a personalized cardiac regeneration system that allows to study the interface of the regenerated cardiac tissue with the blood circulation, in a three-dimensional innovative system. In detail, an organ-on-a-chip with two chambers, one upper and one lower, will be built, put in
communication through a membrane represented by the animal myocardium which is decellularized in order to create a complete and completely natural scaffold. The patient's oral cavity will be biopsied to obtain mesenchymal stem cells which will be in part reprogrammed to
pluripotency (iPS) and then differentiated into cardiomyocytes (put in the upper chamber of the organ-on-a-chip) and in part differentiated in endothelial cells (put in the lower chamber of the organ-on-a-chip) . At the same time, the decellularization of the cardiac tissue from an animal
already sacrificed for other reasons (e.g. slaughter animals) will be carried out and it will be used to create the organ-on-a-chip's membrane. This system will therefore allow to study the functionality of the myocardium in a customized three-dimensional in vitro model, since it will
be ad hoc for the patient having introduced the patient's own cells, which will be able to mimic in a much more efficient way, thanks also to the innovative membrane, than the current organ -on-a-chip functionality of the organ and tissue interfaces. This will have an important implication
especially in clinical application since it will allow to obtain customized transplant personalized grafts perfectly functioning for the replacement of necrotic portion of the heart of patients, as well as reliable models for the study of cardiovascular pathological situations ad hoc for patients.